Publications

Selected Peer-Reviewed Publications (2008-Current)

Flora C, Olesnavich M, Zuo Y, Sandford E, Madhukar R, Rozwadowski M, Sugur K, Ly A, Canbaz AA, Shedeck A, Li G, Geer MJ, Yanik GA, Ghosh M, Frame DG, Bonifant CL, Jain T, Knight JS, Choi SW, Tewari M. Longitudinal plasma proteomics in CAR T-cell therapy patients implicates neutrophils and NETosis in the genesis of CRS. Blood Adv. 2024 Mar 26;8(6):1422-1426. doi:10.1182/bloodadvances.2023010728. PMID38266157
Bhambhani C, Kang Q, Hovelson DH, Sandford E, Olesnavich M, Dermody SM, Wolfgang J, Tuck KL, Brummel C, Bhangale AD, He K, Gutierrez MG, Lindstrom RH, Liu CJ, Tuck M, Kandarpa M, Mierzwa M, Casper K, Prince ME, Krauss JC, Talpaz M, Henry NL, Giraldez MD, Ramnath N, Tomlins SA, Swiecicki PL, Brenner JC, Tewari M. ctDNA transiting into urine is ultrashort and facilitates noninvasive liquid biopsy of HPV+ oropharyngeal cancer. JCI Insight. 2024 Feb 8;9(6):e177759. doi: 10.1172/jci.insight.177759.
PMID38516891 
Bhambhani C, Sandford E, Haring CT, Brummel C, Tuck KL, Olesnavich M, Bhangale AD, Walline HM, Dermody SM, Spector ME, Chinn SB, Casper K, Mierzwa M, Swiecicki PL, Chad Brenner J, Tewari M. Development of a high-performance multi-probe droplet digital PCR assay for high-sensitivity detection of human papillomavirus circulating tumor DNA from plasma. Oral Oncol. 2023 Aug;143:106436. doi: 10.1016/j.oraloncology.2023.106436. Epub 2023 Jun 1. PMID37269557
Nessle CN, Flora C, Sandford E, Choi SW, Tewari M. High-frequency temperature monitoring at home using a wearable device: A case series of early fever detection and antibiotic administration for febrile neutropenia with bacteremia. Pediatr Blood Cancer. 2022 Sep;69(9):e29835. doi: 10.1002/pbc.29835. Epub 2022 Jun 23. PMID35735223
Dermody SM, Bhambhani C, Swiecicki PL, Brenner JC, Tewari M. Trans-Renal Cell-Free Tumor DNA for Urine-Based Liquid Biopsy of Cancer. Front Genet. 2022. Apr 27;13:879108. doi: 10.3389/fgene.2022.879108. PMID35571046
Mayer C, Tyler J, Fang Y, Flora C, Frank E, Tewari M, Choi SW, Sen S, Forger DB. Consumer-grade wearables identify changes in multiple physiological systems during COVID-19 disease progression. Cell Rep Med. 2022 Apr 19;3(4):100601. doi: 10.1016/j.xcrm.2022.100601. eCollection 2022 Apr 19. PMID35480626 
Cao Y, Haring CT, Brummel C, Bhambhani C, Aryal M, Lee C, Heft Neal M, Bhangale A, Gu W, Casper K, Malloy K, Sun Y, Shuman A, Prince ME, Spector ME, Chinn S, Shah J, Schonewolf C, McHugh JB, Mills RE, Tewari M, Worden FP, Swiecicki PL, Mierzwa M, Brenner JC. Early HPV ctDNA Kinetics and Imaging Biomarkers Predict Therapeutic Response in p16+ Oropharyngeal Squamous Cell Carcinoma. Clin Cancer Res. 2022 Jan 15;28(2):350-359. doi: 10.1158/1078-0432.CCR-21-2338. Epub 2021 Oct 26. PMID34702772 
Dimcheff DE, Valesano AL, Rumfelt KE, Fitzsimmons WJ, Blair C, Mirabelli C, Petrie JG, Martin ET, Bhambhani C, Tewari M, Lauring AS. Severe Acute Respiratory Syndrome Coronavirus 2 Total and Subgenomic RNA Viral Load in Hospitalized Patients. J Infect Dis. 2021. Oct 28;224(8):1287-1293. doi: 10.1093/infdis/jiab215. PMID33870434 
Haring CT, Brummel C, Bhambhani C, Jewell B, Neal MH, Bhangale A, Casper K, Malloy K, McLean S, Shuman A, Stucken C, Rosko A, Prince M, Bradford C, Eisbruch A, Mierzwa M, Tewari M, Worden FP, Swiecicki PL, Spector ME, Brenner JC. Implementation of human papillomavirus circulating tumor DNA to identify recurrence during treatment de-escalation. Oral Oncol. 2021 Oct;121:105332. doi: 10.1016/j.oraloncology.2021.105332. Epub 2021 Jun 14. PMID34140235 
Flora C, Tyler J, Mayer C, Warner DE, Khan SN, Gupta V, Lindstrom R, Mazzoli A, Rozwadowski M, Braun TM, Ghosh M, Forger DB, Choi SW, Tewari M. High-frequency temperature monitoring for early detection of febrile adverse events in patients with cancer. Cancer Cell. 2021 Sep 13;39(9):1167-1168. doi: 10.1016/j.ccell.2021.07.019. Epub 2021 Aug 12. PMID34388378 
Chang AE, Golob JL, Schmidt TM, Peltier DC, Lao CD, Tewari M. Targeting the Gut Microbiome to Mitigate Immunotherapy-Induced Colitis in Cancer. Trends Cancer. 2021 Jul;7(7):583-593. doi: 10.1016/j.trecan.2021.02.005. Epub 2021 Mar 16. PMID33741313
​Haring CT, Bhambhani C, Brummel C, Jewell B, Bellile E, Heft Neal ME, Sandford E, Spengler RM, Bhangale A, Spector ME, McHugh J, Prince ME, Mierzwa M, Worden FP, Tewari M, Swiecicki PL, Brenner JC. Human papilloma virus circulating tumor DNA assay predicts treatment response in recurrent/metastatic head and neck squamous cell carcinoma. Oncotarget. 2021 Jun 22;12(13):1214-1229. doi: 10.18632/oncotarget.27992. eCollection 2021 Jun 22. PMID34194620 
​Cislo C, Clingan C, Gilley K, Rozwadowski M, Gainsburg I, Bradley C, Barabas J, Sandford E, Olesnavich M, Tyler J, Mayer C, DeMoss M, Flora C, Forger DB, Cunningham JL, Tewari M, Choi SW. Monitoring beliefs and physiological measures in students at risk for COVID-19 using wearable sensors and smartphone technology: Protocol for a mobile health study. JMIR Res Protoc. 2021 Jun 4;10(6):e29561. doi: 10.2196/29561. Online ahead of print. PMID34115607 
​Clingan CA, Dittakavi M, Rozwadowski M, Gilley KN, Cislo CR, Barabas J, Sandford E, Olesnavich M, Flora C, Tyler J, Mayer C, Stoneman E, Braun T, Forger DB, Tewari M, Choi SW. Monitoring Health Care Workers at Risk for COVID-19 Using Wearable Sensors and Smartphone Technology: Protocol for an Observational mHealth Study. JMIR Res Protoc. 2021 May 12;10(5):e29562. doi:10.2196/29562. PMID3945497 
Song Y, Zhao J, Cai T, Stephens A, Su SH, Sandford E, Flora C, Singer BH, Ghosh M, Choi SW, Tewari M, Kurabayashi K. Machine learning-based cytokine microarray digital immunoassay analysis. Biosens Bioelectron. 2021 May 15;180:113088. doi: 10.1016/j.bios.2021.113088. Epub 2021 Feb 20. PMID33647790
Tewari M. Discovery of Circulating, Cell-Free MicroRNAs: Fundamental Science Forges a New Path for Biomarker Discovery. Clin Chem. 2020 Mar 1;66(3):493-494. doi: 10.1093/clinchem/hvz034. PMID32109305 
He K, Wu Z, Fujiwara H, Whitesall S, Zajac C, Choi SW, Reddy P, Tewari M. Computational Analysis of Continuous Body Temperature Provides Early Discrimination of Graft-versus-Host Disease in Mice. Blood Advances. 2019 Dec 10;3(23):3977-3981. doi: 10.1182/bloodadvances.2019000613. PMID31809535
Giraldez MD, Spengler RM, Etheridge A, Goicochea AJ, Tuck M, Choi SW, Galas DJ, Tewari M​. Phospho-RNA-seq: a modified small RNA-seq method that reveals circulating mRNA and lncRNA fragments as potential biomarkers in human plasma. EMBO J. 2019 June. doi:10.15252/embj.2019101695. PMID31053596
He K, Fujiwara H, Zajac C, Sandford E, Reddy P, Choi SW, Tewari M. A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR. Scientific Reports. 2019 Apr. doi:10.1038/s41598-019-41753-6. PMID30944341
Johnson-Buck A, Li J, Tewari M, Walter NG. A guide to nucleic acid detection by single-molecule kinetic fingerprinting. Methods. 2019 Jan. doi: 10.1016/j.ymeth.2018.08.002. PMID30099084
Hayward SL, Lund PE, Kang Q, Johnson-Buck A, Tewari M, Walter NG. Ultra-specific and Amplification-free Quantification of Mutant DNA by Single-molecule Kinetic Fingerprinting. Journal of the American Chemical Society. 2018 Sep 19. doi: 10:1021/jacs.8b06685. PMID30125495
Giraldez MD, Spengler RM, Etheridge A, Godoy PM, Barczak AJ, Srinivasan S, De Hoff PL, Tanriverdi K, Courtright A, Lu S, Khoory J, Rubio R, Baxter D, Driedonks TAP, Buermans HPJ, Nolte-'t Hoen ENM, Jiang H, Wang K, Ghiran I, Wang YE, Van Keuren-Jensen K, Freedman JE, Woodruff PG, Laurent LC, Erle DJ, Galas DJ, Tewari M. Comprehensive multi-center assessment of small RNA-seq methods for quantitative miRNA profiling. Nat Biotechnol. 2018 Sep. doi: 10.1038/nbt.4183. PMID30010675​
Hovelson DH, Liu CJ, Wang Y, Kang Q, Henderson J, Gursky A, Brockman S, Ramnath N, Krauss JC, Talpaz M, Kandarpa M, Chugh R, Tuck M, Herman K, Grasso CS, Quist MJ, Feng FY, Haakenson C, Langmore J, Kamberov E, Tesmer T, Husain H, Lonigro RJ, Robinson D, Smith DC, Alva AS, Hussain MH, Chinnaiyan AM, Tewari M, Mills RE, Morgan TM, Tomlins SA. Rapid, ultra low coverage copy number profiling of cell-free DNA as a precision oncology screening strategy. Oncotarget. 2017 Sep 22;8(52):89848-89866. doi: 10.18632/oncotarget.21163. eCollection 2017 Oct 27. PMID29163793​
Husain H, Melnikova VO, Kosco K, Woodward B, More S, Pingle SC, Weihe E, Park BH, Tewari M, Erlander MG, Cohen E, Lippman SM, Kurzrock R. Monitoring Daily Dynamics of Early Tumor Response to Targeted Therapy by Detecting Circulating Tumor DNA in Urine. Clin Cancer Res. 2017 Aug 15;23(16):4716-4723. doi: 10.1158/1078-0432.CCR-17-0454. Epub 2017 Apr 18. PMID28420725 ​​
Chevillet JR, Khokhlova TD, Giraldez MD, Schade GR, Starr F, Wany YN, Gallichotte EN, Wang K, Hwang JH, Tewari M. Release of Cell-free MicroRNA Tumor Biomarkers into the Blood Ciculation with Pulsed Focused Ultrasound: A Noninvasive Anatomically Localized, Molecular Liquid Biopsy. Radiology. 2017 April;283(1):158-167. doi: 10.1148/radiol.2016160024. Epub 2016 Nov 1. PMID27802108​
Conley A, Miniciacchi VR, Lee DH,Kundsen BS, Karlan BY, Citrigno L, Viglietto G, Tewari M, Freeman MR, Demichelis F, Di Vizio D. High-throughput sequencing of two populations of extracellular vesicles provides an mRNA signature that can be detected in the circulation of breast cancer patients. RNA Biol. 2017 Mar 4; 14(3): 305-316. doi: 10.1080/15476286.2016.1259061 Epub 2016 Nov 18. PMID27858503​
Kang Q, Henry NL, Paoletti C, Jiang H, Vats P, Chinnaiyan AM, Hayes DF, Merajver SD, Rae JM, Tewari M. Comparative analysis of circulating tumor DNA stability In K3EDTA, Streck, and CellSave blood collection tubes. Clin Biochem. 2016 Apr 27. pii: S0009-9120(16)30040-6. doi: 10.1016/j.clinbiochem.2016.03.012. Epub ahead of print. PMID27129799​
Kang Q, Parkin B, Giraldez MD, Tewari M. Mutant DNA quantification by digital PCR can be confounded by heating during DNA fragmentation. Biotechniques. 2016 Apr 1;60(4):175-85. doi: 10.2144/000114401. eCollection 2016. PMID27071606​
Johnson-Buck A, Su X, Giraldez MD, Zhao M, Tewari M, Walter NG. Kinetic fingerprinting to identify and count single nucleic acids. Nature Biotechnology, 2015 Jul;33(7):730-2. PMID26098451
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Chevillet JC, Kang Q, Ruf IK, Briggs HA, Vojtech LN, Hughes SM, Cheng HH, Arroyo JD, Meredith EK, Gallichotte EN, Pogosova-Agadjanyane EL, Morrissey C, Stirewalt DL, Hladik F, Yu EY, Higano CS, Tewari M. Quantitative and stoichiometric analysis of the microRNA content of exosomes. PNAS (USA), 2014 Oct 14;111(41):14888-93. doi: 10.1073/pnas.1408301111. Epub 2014 Sep 29. PMID2526762 ​​
Vojtech L, Woo S, Hughes S, Levy C, Ballweber L, Sauteraud RP, Strobl J, Westerberg K, Gottardo R, Tewari M, Hladik F. Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions. Nucleic Acids Res. 2014; 42(11):7290-304. doi: 10.1093/nar/gku347. Epub 2014 May 16.  PMID24838567​
Arroyo JD, Gallichotte EN, Tewari M. Systematic design and functional analysis of artificial microRNAs. Nucleic Acids Res. 2014; 42(9):6064-77. doi: 10.1093/nar/gku171. Epub 2014 Mar 5. PMID24598260 ​
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Nair VS, Pritchard CC, Tewari M, Ioannidis JP. Design and Analysis for Studying microRNAs in Human Disease: A Primer on -Omic Technologies. Am J Epidemiol. 2014; 15;180(2):140-52. PMID24966218
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Kullolli M, Knouf E, Arampatzidou M, Tewari M, Pitteri SJ. Intact MicroRNA Analysis Using High Resolution Mass Spectrometry. J Am Soc Mass Spectrom. 2014 Jan; 25(1):80-7. doi: 10.1007/s13361-013-0759-x. Epub 2013 Oct 31. PMID24174127 ​​
Robins HS, Ericson N, Guenthoer J, O’Briant KC, Tewari M, Drescher CW, Bielas JH. Digital quantification of tumor infiltrating lymphocytes. Sci Transl Med. 2013 Dec 4; 5(214):214ra169. doi: 10.1126/scitranslmed.3007247 PMID24307693​
Emerson RO, Sherwood AM, Rieder MJ, Guenthoer J, Williamson DW, Carlson CS, Drescher CW, Tewari M, Bielas JH, Robins HS. High-throughput sequencing of T cell receptors reveals a homogeneous repertoire of tumor-infiltrating lymphocytes in ovarian cancer. J Pathol. 2013 Dec; 231(4):4330-40. doi: 10.1002/path.4260.  PMID24027095​
Hindson CM, Chevillet JR, Briggs H, Gallichotte E, Ruf IK, Hindson BJ, Vessella RL, Tewari M. Absolute quantification of low abundance targets by droplet digital PCR versus analog real-time PCR. Nat Methods. 2013 Oct;10(10):1003-5. doi: 10.1038/nmeth.2633. Epub 2013 Sep 1. PMID23995387​
Cheng HH, Mitchell PS, Kroh EM, Dowell AE, Chéry L, J, Nelson PS, Vessella RL, Knudsen BS, Chinnaiyan AM, Pienta KJ, Morrissey C, Tewari M. Circulating microRNA Profiling Identifies a Subset of Metastatic Prostate Cancer Patients with Evidence of Cancer-Associated Hypoxia. PLoS One. 2013 Jul 30;8(7):e69239. doi: 10.1371/journal.pone.0069239. Print 2013. PMID23935962 ​
Knouf E, Wyman S and Tewari M. The human TUT1 nucleotidyl transferase as a global regulator of microRNA abundance. PLoS One. 2013 Jul 18;8(7):e69630. doi: 10.1371/journal.pone.0069630. Print 2013. PMID23874977 ​
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Cheng HH, Yi HS, Kim Y, Kroh EM, Chien JW, Eaton KD, Goodman MT, Tait JF, Tewari M and Pritchard CC. Plasma processing conditions substantially influence circulating microRNA biomarker levels. PLoS One. 2013 Jun 7;8(6):e64795. doi: 10.1371/journal.pone.0064795. Print 2013. PMID23762257   ​​
Pritchard CC, Cheng HC and Tewari M. MicroRNA profiling: approaches and considerations. Nat Rev Genet.  2012 Apr 18;13(5) 358-69. doi: 10.1038/nrg3198. Review. PMID22510765
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Pritchard CC, Kroh EM, Wood B, Arroyo JD, Dougherty KJ, Miyaji MM, Tait JF and Tewari M. Blood cell origin of circulating microRNAs: a cautionary note for cancer biomarker studies. Cancer Prev Res (Phila).  2012 Mar; 5(3):492-7. doi: 10.1158/1940-6207.CAPR-11-0370. Epub 2011 Dec 12. PMID22158052  ​
Knouf EC, Garg KS, Arroyo JD, Correa Y, Sarkar D, Parkin RK, Wurz K, O’Briant KC, Godwin AK, Urban ND, Ruzzo WL, Gentleman R, Drescher CW, Swisher EM, Tewari M. An integrative genomic approach identifies p73 and p63 as activators of miR-200 microRNA family transcription. Nucleic Acids Res. 2012 Jan;40(2):499-510. doi: 10.1093/nar/gkr731. Epub 2011 Sep 14.  PMID21917857 ​​
Wyman SK, Knouf EC, Parkin RK, Lin DW, Dennis L, Krouse MA, Webster PJ, Tewari M. Post-transcriptional generation of miRNA variants by multiple nucleotidyl transferases contributes to miRNA transcriptome complexity. Genome Res. 2011 Sep;21(9):1450-61. doi: 10.1101/gr.118059.110. Epub 2011 Aug 3.  PMID21813625 ​​
Arroyo JD, Chevillet JR, Kroh EM, Ruf IK, Pritchard CC, Gibson DF, Mitchell PS, Bennett CF, Pogosova-Agadjanyan EL, Stirewalt DL, Tait JF, Tewari M.  Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma.  Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):5003-8. doi: 10.1073/pnas.1019055108. Epub 2011 Mar 7. PMID21383194  ​
Kroh EM, Parkin RK, Mitchell PS, Tewari M. Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR). Methods. 2010 Apr;  50(4):298-301. doi: 10.1016/j.ymeth.2010.01.032. Epub 2010 Feb 8. Erratum in: Methods. 2010 Nov; 52(3):268. PMID20146939​​​
Bendoraite A, Knouf EC, Garg KS, Parkin RK, Kroh EM, O’Briant KC, Ventura AP, Godwin AK, Karlan BY, Drescher CW, Urban N, Knudsen BS, Tewari M.  Regulation of miR-200 family microRNAs and ZEB transcription factors in ovarian cancer: Evidence supporting a mesothelial-to-epithelial transition. Gynecol Oncol. 2010 Jan;116(1):117-25. doi: 10.1016/j.ygyno.2009.08.009. Epub 2009 Oct 24. PMID19854497     ​​
Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O'Briant KC, Allen A, Lin DW, Urban N, Drescher CW, Knudsen BS, Stirewalt DL, Gentleman R, Vessella RL, Nelson PS, Martin DB, ​Tewari M. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA 2008 Jul 29;105(30):10513-8. doi: 10.1073/pnas.0804549105. Epub 2008 Jul 28. PMID18663219​

Non Peer-Reviewed Publications

  • Tewari M. A functional extracellular transcriptome in animals? Implications for biology, disease and medicine. Genome Biol 16:47, 2015.
  • Tewari M and Vidal M. RNAi on the apoptosis TRAIL: the mammalian cell genetic screen comes of age. Dev Cell 5:534-535, 2003.
  • Tewari M and Dixit VM. Recent advances in tumor necrosis factor and CD40 signaling. Curr Opin Genet Dev 6:39-44, 1996.
  • Tewari M. Nuclear magnetic resonance microscopy. Case Engineering and Science Review 31(1):12-19, 1989.

Book Chapters

  • Giraldez MD, Chevillet JR, and Tewari M. Droplet digital PCR for absolute quantification of extracellular microRNAs in plasma and serum: quantification of the cancer biomarker hsa-miR-141. METHODS IN MOLECULAR BIOLOGY. In press, Springer, 2018.Russo IH, Tewari M, and Mitchell PS and Tewari M. Circulating MicroRNAs in Cancer. In: Extracellular Nucleic Acids, a volume of Nucleic Acids and Molecular Biology Series, Kikuchi, Rykova eds., Springer-Verlag, 2010.  
  • LeMeur N, Lawrence M, Bar M, Tewari M, and Gentleman R. R and Bioconductor Packages in Bioinformatics: Toward Systems Biology. In: Statistical Bioinformatics for Biomedical and Life Science Researchers, Lee JK ed., Wiley-Blackwell, 2010.   
  • Rao A and Tewari M.  Systems Biology Approaches to TGF-ß/Smad Signaling. In: Smad Signal Transduction: Smads in Proliferation, Differentiaton and Disease. Volume 5, Proteins and Cell Regulation, ten Dijke P, Heldin CH, eds., Springer, 2006.    
  • Tewari M and Dixit VM.  Signaling Through Members of the TNF-R Family. In: Modular Texts in Molecular and Cell Biology, Purton M and Heldin C, eds. Chapman and Hall: London, 1996.   
  • Russo, IH, Tewari M, and Russo J. Morphology and Development of the Rat Mammary Gland. In: Integument and Mammary Glands, Jones, Konishi, Mohr, eds. Springer-Verlag: Berlin, 1989.